WebDec 2, 2024 · Transfection was carried out by using Lipotransfectamine 3000. Dual-Luciferase Report Assay (Promega) was performed after transfection for 48 h. Chromatin immunoprecipitation assay Briefly, PANC-1 or BxPC-3 cells were cross-linked with 1% PFA. After sonicating and pre-clearing, cells were incubated with appropriate antibodies. WebFeb 4, 2016 · Panc1 cells (5 × 10 4) were seeded on 24-well plates 1 day before transfection. The cells were transfected with 0.45μg reporter plasmid (pGL3-SOCS3) or DNMT1 plasmid at the same time using Lipofectamine 2000 transfection reagent. 0.05μg Renilla luciferase plasmid phRL-tk was co-transfected to normalize the transfection …
PANC1 Transfection Kit (Non-endocrine Pancreatic Cn) InVivo and Cel…
WebCommonly known as G418 or G-418, Gibco Geneticin reagent is an aminoglycoside related to gentamicin and is commonly used as a selective agent for eukaryotic cells. As an analog of neomycin sulfate, it interferes with the function of 80S ribosomes and protein synthesis in eukaryotic cells. WebMar 17, 2024 · Normal control and EGR1-overexpressed PANC1 cells were constructed via stable transfection of the aforementioned lentivirus. The model of liver metastasis of pancreatic cancer was established by injecting cancer cells (1 × 10 6 cells/50 μL) to the spleen of BALB/c-nude mice (6 weeks, male). culligan whole house carbon filter
Targeting E2 ubiquitin-conjugating enzyme UbcH5c by small …
WebMix RNP complexes with PANC-1 CRISPR transfection reagent. For Cas9 protein with an NLS sequence and attached sgRNA, combine 40 μl serum-free medium, 5.5 μl transfection reagent, and 750 ng of RNP complexes. a. If using a repair template, add 50 ng during this step. The repair template must also be encapsulated to ensure high transfection ... WebNov 4, 2024 · PANC1 and MIA cells were lysed using Trizol regent. Total RNA was extracted from the cells and 1 μg of the RNA was subjected to reversed transcription reaction with M-MLV reverse transcriptase (Promega). Quantification of the cDNA (1/50 of the reversed product) was performed in triplicate with SYBR master mixture on the … WebAug 20, 2024 · (A) Panc1 cells were transfected twice with 25 nM each of Co siRNAs specific for RAC1B and PAR2, 25 nM each of RAC1B siRNA + Co siRNA specific for PAR2, or 25 nM each of RAC1B siRNA + PAR2 siRNA. Forty-eight hours after the second transfection, these Panc1-RAC1B-KD cells were subjected to RNA isolation and qPCR … culligan whole house filters