WebThe mTOR, autophagy-related proteins and apoptosis-related proteins were detected by Western blot or real-time PCR in vivo and vitro. MPC5 cells were exposed to high glucose (HG, 30mM) for 12h to simulate podocyte injury in DN. MPC5 cells were treated by serum containing YNJ with different dosages. Cell activities and apoptosis were ... WebThese signals induce macroautophagy, either via inhibition of mTOR or by AMPK activation of the ULK1/2 kinase complex. mTOR activity negatively regulates autophagy, which may involve AMP kinase induced phosphorylation of mTOR on specific sites. Autophagy can also be induced in mTOR-independent manner by lowering the levels of myo-inositol-1, 4 ...
Molecular regulation of autophagy machinery by mTOR…
WebIndirizzo Via Giovanni Amendola 122/O - 70126 Bari. Email [email protected]. Tel. +39 080 592 9142/41 Web8 iun. 2010 · GPP78 is a potent NAMPT inhibitor. Nampt inhibition induces a potent cytotoxic activity against multiple myeloma cell lines in vitro and in vivo. Moreover, Nampt inhibition also triggers a marked increase in autophagy. NAMPT inhibitors are beneficial in inflammatory animal models of arthritis and endotoxic shock as well as in autoimmune ... blank forms for a will
Transcriptional regulation of autophagy and its implications in …
WebAutophagy level was increased in atrophied skeletal muscle after CLP, and NRG-1β could inhibit autophagy through the Akt/mTOR pathway. The levels of ULK-1, p-Beclin-1, Beclin-1, and the ratio of LC3II/I in the tibialis anterior muscle were increased in the Sep group (P < 0.001 or P < 0.01), while the content of p62 was decreased (P < 0.001). WebAcum 2 zile · Macroautophagy/autophagy is a conserved catabolic pathway that is vital for maintaining cell homeostasis and promoting cell survival under stressful conditions. ... WebRapamycin enhanced liver autophagy induction at the reperfusion stage. Dual mammalian (mechanistic) target of rapamycin (mTOR)1/2 inhibitor Torin 1, despite its ability to induced autophagy, failed to protect livers from IRI. The treatment with RPM, but not Torin 1, resulted in the enhanced activation of the mTORC2-Akt signaling pathway ... blank form medical release