Dm-nitrophen
WebDM-nitrophen is a photolabile derivative of EDTA and therefore functions as either caged Ca(2+) or caged Mg(2+). Several papers have been published recently which use the AM ester to load intact cells with DM-nitrophen; under these conditions it is caged Mg(2+). … WebIn experiments with resealed human erythrocyte ghosts, irradiation of ghosts containing the DM-nitrophen-Ca2+ complex activates a Ca2+-dependent K+ efflux pathway, which is not observed in the absence of illumination. DM-nitrophen is sufficiently stable and …
Dm-nitrophen
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WebLarger [Ca 2+] i jumps elicited by photolysis of DM-nitrophen evoke greater I Ca inactivation, but no potentiation, perhaps because of the more transient rise in [Ca 2+] i when DM-nitrophen is photolyzed. DM-nitrophen loaded with magnesium in the … WebSep 2, 1999 · Laser-flash photolysis of DM-nitrophen has been shown to result in a rapid concentration jump of Ca 2+ (τ<30 µs; ref. 14) that was reflected in an immediate increase in inward I Na/Ca in our ...
WebDM-NITROPHEN Reagent, Tetrasodium Salt. Synonym(s): DM-NITROPHEN Reagent, Tetrasodium Salt. Empirical Formula (Hill Notation): C 18 H 19 N 3 O 12 · 4Na. Molecular Weight: 561.32. Compare Product No. Description SDS Pricing; 317210: Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon … WebDec 28, 2011 · It is furthermore compatible with DM-nitrophen since the excitation wavelength of nm also prevents unwanted uncaging since it does not photolyze DM-nitrophen . Second, its K d (345 nM) makes fluo-4 …
WebJul 1, 2014 · We have used UV flash photolysis of DM-nitrophen in combination with model-based analysis of Oregon Green 488 BAPTA-5N fluorescence transients to study the kinetics of Ca(2+) binding to calbindin ... WebNitrophenyl-EGTA and DM-nitrophen are Ca2+ cages that release Ca2+ when cleaved upon illumination with near-ultraviolet light. Laser photolysis of nitrophenyl-EGTA produced transient intermediates that decayed biexponentially with rates of 500,000 s-1 and …
WebPrepare a DM-nitrophen stock solution by dissolving DM-nitrophen tetrasodium salt (1-[2-nitro-4,5-dimethoxyphenyl]-1,2-diaminoethane-N,N,N',N'-tetraacetic acid, 4Na +; Merck) (nominally 100 m m) in sterile ddH 2 O. Protect the solution from ambient light whenever …
Web6. Photolysis of DM-nitrophen at lower light intensities produced a slower rate of transmitter release. 7. Brief light exposures, i.e. those which photolysed 5-20% of the DM-nitrophen, resulted in a rapid decay of postsynaptic responses on extinguishing the light, due to … chademo v2h ガイドラインWebCaged-Ca(2+) compounds such as nitrophenyl-EGTA (NP-EGTA) and DM-nitrophen (DMn) are extremely useful in biological research, but their use in live cells is hampered by cytoplasmic [Mg(2+)]. We determined the properties of Ca(2+) release from NP-EGTA … chads2 vascスコア ガイドラインWebFeb 2, 1999 · Regulated exocytosis triggered by the photolysis of a caged Ca 2+ compound, DM-nitrophen, was investigated by patch-clamp capacitance measurements in two clones of PC12, the first wild-type and the second (PC12-27) defective of both types of classical secretory vesicles together with the neuronal-type receptors for the attachment proteins … chads2 スコアWebDM-NITROPHEN™ Reagent, Tetrasodium Salt - Calbiochem Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis. - Find MSDS or SDS, a COA, data sheets and more information. chadoxl ncov- 19 ワクチンWebJul 1, 1990 · Competing binding reactions cause the amount of Ca2+ released by DM-nitrophen photolysis to depend on the concentrations of DM-nitrophen, total Ca2+, Mg+, ATP and native cytoplasmic Ca2+ buffer. Measurements of presynaptic [Ca2+] changes by co-injection of the fluorescent indicator dye Fura-2 show that DM-nitrophen photolysis … chadox1-s ワクチンWebOct 16, 2012 · DM-nitrophen-Ca, Alexa dyes, and the Ca indicator OGB-5N were applied intracellularly by whole-cell recording. Photolysis of DM-nitrophen was with a 1-, 3-, or 5-μm laser spot at 405 nm. Fluorescence excitation was with light-emitting diode light sources, and images were recorded with a CCD camera. chads2スコア 1点WebDec 15, 2016 · DMNP-EDTA (also known as DM-Nitrophen™) is a caged Ca 2+ chelator. Upon photolysis, the Kd for Ca 2+ increases from 5 nM … chademo急速充電システム